In Vitro Biofilm Modelling

Experimental recreation of biofilms in the laboratory allows us to develop reproducible environments for the testing of isolated variables, whether that be for growth, infection, disruption, or therapeutics. From simple mono-species biofilms to investigate individual bacterial responses, multi-species models for synergistic/antagonistic responses, through to the network of complex interactions within entire communities. Often collaborative in nature, cross-discipline modelling of biofilms enables us to harness the expertise from biological sciences, dentistry, physics, engineering, chemistry, food and environmental sciences to address multifactorial issues in greater depth. 

Researchers across our faculties have expertise in a variety of biofilm modelling techniques, including: 

• Hydroxyapatite-coated peg systems to imitate dental environments, for the investigation of the efficacy of antimicrobial peptides, toothpaste components or probiotics. 
• Uniquely designed microfluidics devices to automate controlled biofilm production and allow continuous monitoring of growth without disruption. 

• Chemostat-based cascade weir systems of the human gut. By replicating the environmental condition within the colon, such as pH and nutrient gradients, the impact of infection, probiotics and metabolism has been investigated. 
• In-situ enamel devices attached to the teeth of volunteers for the harvesting of natural oral biofilms. 
• Co-culture systems to study the interactions between eukaryotic and prokaryotic cells. By exploring two and three-dimensional cell culture, in conjunction with biofilms grown on implant materials, the multifaceted elements of dental implant integration, biocompatibility and infection are being assessed.